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Electrospinning PCL Scaffolds Manufacture for Three-Dimensional Breast Cancer Cell Culture

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dc.contributor.author Rabionet Díaz, Marc
dc.contributor.author Yeste Oliveras, Marc
dc.contributor.author Puig i Miquel, Teresa
dc.contributor.author Ciurana, Quim de
dc.date.issued 2017-08-01
dc.identifier.issn 2073-4360
dc.identifier.uri http://hdl.handle.net/10256/14368
dc.description.abstract In vitro cell culture is traditionally performed within two-dimensional (2D) environments, providing a quick and cheap way to study cell properties in a laboratory. However, 2D systems differ from the in vivo environment and may not mimic the physiological cell behavior realistically. For instance, 2D culture models are thought to induce cancer stem cells (CSCs) differentiation, a rare cancer cell subpopulation responsible for tumor initiation and relapse. This fact hinders the development of therapeutic strategies for tumors with a high relapse percentage, such as triple negative breast cancer (TNBC). Thus, three-dimensional (3D) scaffolds have emerged as an attractive alternative to monolayer culture, simulating the extracellular matrix structure and maintaining the differentiation state of cells. In this work, scaffolds were fabricated through electrospinning different poly("-caprolactone)-acetone solutions. Poly("-caprolactone) (PCL) meshes were seeded with triple negative breast cancer (TNBC) cells and 15% PCL scaffolds displayed significantly (p < 0.05) higher cell proliferation and elongation than the other culture systems. Moreover, cells cultured on PCL scaffolds exhibited higher mammosphere forming capacity and aldehyde dehydrogenase activity than 2D-cultured cells, indicating a breast CSCs enrichment. These results prove the powerful capability of electrospinning technology in terms of poly("-caprolactone) nanofibers fabrication. In addition, this study has demonstrated that electrospun 15% PCL scaffolds are suitable tools to culture breast cancer cells in a more physiological way and to expand the niche of breast CSCs. In conclusion, three-dimensional cell culture using PCL scaffolds could be useful to study cancer stem cell behavior and may also trigger the development of new specific targets against such malignant subpopulation cat
dc.description.sponsorship This work was supported partially by Spanish grants from Fundación Ramón Areces, Instituto de Salud Carlos III (PI1400329) and Ministerio de Economía Y Competitividad (DPI2013-45201-P; RYC-2014-15581), and the support of the Catalonian government (2014SGR00868). The authors are grateful for the financial support from the University of Girona (MPCUdG2016/036) cat
dc.format.mimetype application/pdf cat
dc.language.iso eng cat
dc.publisher MDPI (Multidisciplinary Digital Publishing Institute) cat
dc.relation MINECO/PE 2014-2016/DPI2013-45201-P cat
dc.relation.isformatof Reproducció digital del document publicat a: https://doi.org/10.3390/polym9080328 cat
dc.relation.ispartof Polymers, 2017, vol. 9, núm. 8, p. 328 cat
dc.relation.ispartofseries Articles publicats (D-EMCI) cat
dc.rights Attribution 4.0 Spain *
dc.rights.uri http://creativecommons.org/licenses/by/4.0/es/ *
dc.subject Mama -- Càncer cat
dc.subject Breast -- Cancer cat
dc.subject Materials nanoestructurats cat
dc.subject Nanostructured materials cat
dc.subject Polímers en medicina cat
dc.subject Polymers in medicine cat
dc.subject Nanotecnologia cat
dc.subject Nanotechnology cat
dc.title Electrospinning PCL Scaffolds Manufacture for Three-Dimensional Breast Cancer Cell Culture cat
dc.type info:eu-repo/semantics/article cat
dc.rights.accessRights info:eu-repo/semantics/openAccess cat
dc.embargo.terms Cap cat
dc.type.version info:eu-repo/semantics/publishedVersion cat
dc.identifier.doi https://doi.org/10.3390/polym9080328
dc.contributor.funder Ministerio de Economía y Competitividad (Espanya) cat

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