Quantitative profiling of perfluoroalkyl substances by ultrahigh-performance liquid chromatography and hybrid quadrupole time-of-flight mass spectrometry

Text Complet
Quantitative-profiling.pdf closed access
Sol·licita còpia a l'autor de l'article
En omplir aquest formulari esteu demanant una còpia de l'article dipositat al repositori institucional (DUGiDocs) al seu autor o a l'autor principal de l'article. Serà el mateix autor qui decideixi lliurar una còpia del document a qui ho sol•liciti si ho creu convenient. En tot cas, la Biblioteca de la UdG no intervé en aquest procés ja que no està autoritzada a facilitar articles quan aquests són d'accés restringit.
Compartir
The accurate determination of perfluoroalkyl substances(PFSAs) in water, sediment, fish, meat, and human milk was achieved by ultrahigh-performance liquid chromatography– quadrupole time-of-flight mass spectrometry (UHPLC–QqTOF-MS) with an ABSciex Triple TOF®. A group of 21 PFSAs was selected as target to evaluate the quantitative possibilities. Full scan MS acquisition data allows quantification at relevant low levels (0.1–50 ng L−1 in water, 0.05–2 ng g−1 in sediments, 0.01–5 ng g−1 in fish and meat, and 0.005–2 ng g−1 in human milk depending on the compound). Automatic information dependent acquisition product ion mass spectrometry (IDA-MS/MS) confirms the identity even for those compounds that presented only one product ion. The preparation of a homemade database using the extracted ion chromatogram (XIC) Manager of the software based upon retention time, accurate mass, isotopic pattern, and MS/MS library searching achieves not only the successful identification of PFSAs but also of some pharmaceuticals, such as acetaminophen, ibuprofen, salicylic acid, and gemfibrozid. Mean recoveries and relative standard deviation (RSD) were 67–99% (9–16% RSD) for water, 62–103 % (8– 18 % RSD) for sediment, 60–95 % (8–17 % RSD) for fish, 64–95 % (8–15 % RSD) for meat, and 63–95 % (8–16 %) for human milk. The quantitative data obtained for 60 samples by UHPLC–QqTOF-MS agree with those obtained by LC–MS/MS with a triple quadrupole (QqQ) ​
​Tots els drets reservats